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anti myhc  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti myhc
    Anti Myhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti myhc/product/Developmental Studies Hybridoma Bank
    Average 96 stars, based on 262 article reviews
    anti myhc - by Bioz Stars, 2026-05
    96/100 stars

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    Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and <t>anti-MHC</t> (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).
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    Image Search Results


    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Journal: Frontiers in Nutrition

    Article Title: Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses

    doi: 10.3389/fnut.2026.1722091

    Figure Lengend Snippet: Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Article Snippet: Primary antibodies against myosin heavy chain isoforms were applied overnight at 4 °C (type I (BA-D5), IIa (SC-71), IIx (6H1), or IIb (BF-F3) (all from DSHB, Iowa City, IA, USA)).

    Techniques: Purification, Muscles, Staining, Fluorescence

    Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and anti-MHC (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).

    Journal: Development (Cambridge, England)

    Article Title: Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments

    doi: 10.1242/dev.205047

    Figure Lengend Snippet: Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and anti-MHC (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).

    Article Snippet: Embryos were stained using mouse monoclonal anti-Myosin heavy chain (MHC) antibody (Developmental Studies Hybridoma Bank, A4.1025; RRID:AB_528356) at 1:200 dilution, rat monoclonal anti-mCherry antibody (Invitrogen, M11217 ; RRID:AB_2536611) at 1:500 dilution, rabbit polyclonal anti p-Myosin light chain (p-MLC) antibody (Cell Signaling Technology, 3671; RRID:AB_330248) at 1:500 dilution, rabbit anti-Thrombospondin4b antibody (Thbs4b) (GeneTex, GTX125869; RRID:AB_2885605) at 1:1000 dilution.

    Techniques: Staining, Expressing

    Genetic perturbation of Wnt signaling disrupts muscle attachments and tendon pattern. (A-R) Ventral views of cranial tendons at 48 hpf and 60 hpf Tg(scxa:mCherry) , Tg(scxa:mCherry; hsp70l:dkk1b-GFP) and Tg(scxa:mCherry; hsp70l:dnTCF-GFP) embryos that were heat shocked and immunostained for anti-mCherry (tenocytes), anti-GFP (Wnt responsive cells) and anti-MHC (muscle fibers). Images show muscle attachments in untreated (A,J) and heat-shocked controls (B,K), Tg(scxa:mCherry) and heat-shocked transgenics with perturbed Wnt signaling (D-I, M-R). (C,L) Histograms show distribution of normal, mild and severe muscle attachment defects between control and heat-shocked Tg(scxa:mCherry; hsp70l:dkk1b-GFP) embryos. P -values were calculated using chi-square test of independence. *** P <0.001 (48 hpf, n ≈79; 60 hpf, n ≈76). Scale bars: 30 μm.

    Journal: Development (Cambridge, England)

    Article Title: Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments

    doi: 10.1242/dev.205047

    Figure Lengend Snippet: Genetic perturbation of Wnt signaling disrupts muscle attachments and tendon pattern. (A-R) Ventral views of cranial tendons at 48 hpf and 60 hpf Tg(scxa:mCherry) , Tg(scxa:mCherry; hsp70l:dkk1b-GFP) and Tg(scxa:mCherry; hsp70l:dnTCF-GFP) embryos that were heat shocked and immunostained for anti-mCherry (tenocytes), anti-GFP (Wnt responsive cells) and anti-MHC (muscle fibers). Images show muscle attachments in untreated (A,J) and heat-shocked controls (B,K), Tg(scxa:mCherry) and heat-shocked transgenics with perturbed Wnt signaling (D-I, M-R). (C,L) Histograms show distribution of normal, mild and severe muscle attachment defects between control and heat-shocked Tg(scxa:mCherry; hsp70l:dkk1b-GFP) embryos. P -values were calculated using chi-square test of independence. *** P <0.001 (48 hpf, n ≈79; 60 hpf, n ≈76). Scale bars: 30 μm.

    Article Snippet: Embryos were stained using mouse monoclonal anti-Myosin heavy chain (MHC) antibody (Developmental Studies Hybridoma Bank, A4.1025; RRID:AB_528356) at 1:200 dilution, rat monoclonal anti-mCherry antibody (Invitrogen, M11217 ; RRID:AB_2536611) at 1:500 dilution, rabbit polyclonal anti p-Myosin light chain (p-MLC) antibody (Cell Signaling Technology, 3671; RRID:AB_330248) at 1:500 dilution, rabbit anti-Thrombospondin4b antibody (Thbs4b) (GeneTex, GTX125869; RRID:AB_2885605) at 1:1000 dilution.

    Techniques: Control